Journal: Biology
Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
doi: 10.3390/biology10090934
Figure Lengend Snippet: Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.
Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).
Techniques: Staining